Movie 5

Computer assisted analysis of renal cell migration dynamics can be achieved with the FiZD. Subjection of the time-lapse image stack from the FiZD to computer aided image analysis enabled quantitation of the cell migration dynamics as depicted with the Wind rose plot. The Wind rose plot shows the distribution of magnitude and direction of the cell movements in right half of ureteric bud branch tip for each time point. Length of spokes indicates the proportion of cells moving in a particular direction and thickness of colour bands within a spoke indicates the distribution of speed for these cells.

Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging

Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini, Veli-Pekka Ronkainen, Janne Heikkilä, Ilya Skovorodkin, and Seppo J. Vainio

Development 2017. 144:1113-1117; doi: 10.1242/dev.142950