Movie 3

Internalization of transplanted mesoderm cells.
Confocal image series of internalizing Tg(βActin:Ras-eGFP) mesoderm cells (green) transplanted directly below the EVL at the dorsal germ ring of a Tg(dharma:eGFP);MZoep host embryo, expressing LynTagBFP in all cells to outline the plasma membrane (red) and injected with fluorescent Dextan (blue) to label the interstitial fluid. The transplanted donor cells migrate from the surface to the inside of the germ ring in the host embryo. Frame rate, ~ 2 fps; ~ 4 min/frame. Scale bar, 20 μm.

Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation

S. F. Gabriel Krens, Jim H. Veldhuis, Vanessa Barone, Daniel Čapek, Jean-Léon Maître, G. Wayne Brodland, and Carl-Philipp Heisenberg

Development 2017. 144:1798-1806; doi: 10.1242/dev.144964