Movie 6

Finite Element simulations of ectoderm-mesoderm tissue envelopment using relative interfacial tension ratios obtained from those tissues in the presence of medium with 250 mOsm/L.
When ectoderm and mesoderm aggregates are co-cultured in 250 mOsm/L medium for 5 h, the CellFIT-3D analyses obtained relative interfacial tensions distribution are γ_e-e = 1.00, γ_m-m = 1.16, γ_e-m = 1.32, γ_e-if = 1.52, γ_m-if = 1.41 (see also Fig. 4E). When these relative tensions act in two homotypic model aggregates brought into contact, the mesoderm cell aggregate does not envelope the ectoderm cell aggregate. Frame rate, ~ 30 fps.