Tracking of endogenous mesoderm cell internalization.
Confocal image series of ppl progenitor cell (green) internalization within the dorsal germ ring margin of a Tg(-4gsc:eGFP-Hsa.HRAS) embryo injected with Lyn-TagBFP mRNA to outline the plasma membrane of all cells (red) and Dextran-Rhodamin to label the interstitial fluid (blue). Cell tracks delineate the movements of exemplary ppl (green spheres) and ectoderm (red spheres) progenitor cells during internalization (5 - 6.5 hpf). Frame rate, ~ 2 fps; ~ 2.5 min/frame. Scale bar, 20 μm.