Movie 7

Tracking of endogenous mesoderm cell internalization.
Confocal image series of ppl progenitor cell (green) internalization within the dorsal germ ring margin of a Tg(-4gsc:eGFP-Hsa.HRAS) embryo injected with Lyn-TagBFP mRNA to outline the plasma membrane of all cells (red) and Dextran-Rhodamin to label the interstitial fluid (blue). Cell tracks delineate the movements of exemplary ppl (green spheres) and ectoderm (red spheres) progenitor cells during internalization (5 - 6.5 hpf). Frame rate, ~ 2 fps; ~ 2.5 min/frame. Scale bar, 20 μm.

Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation

S. F. Gabriel Krens, Jim H. Veldhuis, Vanessa Barone, Daniel Čapek, Jean-Léon Maître, G. Wayne Brodland, and Carl-Philipp Heisenberg

Development 2017. 144:1798-1806; doi: 10.1242/dev.144964