Movie 8

Internalization of DN-Rac expressing mesoderm cells.
Confocal image series of ppl progenitor cell (green) internalization in a Tg(-4gsc:eGFP-Hsa.HRAS) embryo (5 - 6.5 hpf), overexpressing DN-Rac to inhibit progenitor cell protrusion formation, Lyn-TagBFP to outline the plasma membrane (red), and Dextran-Rhodamin to label the interstitial fluid (blue). Frame rate, ~ 2 fps; 3 min/frame. Scale bar, 20 μm.

Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation

S. F. Gabriel Krens, Jim H. Veldhuis, Vanessa Barone, Daniel Čapek, Jean-Léon Maître, G. Wayne Brodland, and Carl-Philipp Heisenberg

Development 2017. 144:1798-1806; doi: 10.1242/dev.144964