Movie 2

Wildtype HUVEC vessels were examined by time-lapse microscopy. Transient contacts (arrowhead) and stable connection (arrow) are noted. Insets, high magnification time frames for transient contacts and stable connections. For transient contacts, the frame showing the contact and the frame immediately following (and lacking the contact) are shown for clarity; for stable connections the first frame showing the connection is shown. An additional vessel is present outside the focal plane that was not scored. Scale bar, 25 μm (lower right); time, hrs:mins (lower left).