Movie 6

HUVEC vessels with either wildtype (WT, no cytoplasmic reporter) or a 50/50 mixture of untransfected cytoplasmic-tdTomato and cytoplasmic-eGFP transfected with si-Flt1 were examined by time-lapse microscopy. Stable connection (arrow) is noted. Inset, high magnification first frame showing a stable connection. Scale bar, 25 μm (lower right); time, hrs:mins (lower left).

Blood vessel anastomosis is spatially regulated by Flt1 during angiogenesis

Jessica E. Nesmith, John C. Chappell, Julia G. Cluceru, and Victoria L. Bautch

Development 2017. 144:889-896; doi: 10.1242/dev.145672