3-D image reconstruction of kdrl:mcherry-positive blood vessels (red) and mrc1a:egfp-positive lymphatic vessels and posterior cardinal vein (green) in the trunk of a 13 dpf Tg(mrc1a:egfp)y251, Tg(kdrl:mcherry)y171 double-transgenic zebrafish, showing the fully both superficial/later lymphatics (lateral lymphatic, superficial intersegmental lymphatics) and deep/medial lymphatics (spinal lymphatic, thoracic duct, and collateral cardinal lymphatic). Movie is generated from the same image data as Fig. 6A,N,Q.
Hyun Min Jung, Daniel Castranova, Matthew R. Swift, Van N. Pham, Marina Venero Galanternik, Sumio Isogai, Matthew G. Butler, Timothy S. Mulligan, and Brant M. Weinstein
Development
2017. 144:2070-2081; doi: 10.1242/dev.145755