Supplemental Movie 1

Notch signaling is active in the developing vasculature. Representative time-lapse confocal microscopy of a Tg(kdrl:mCherry); Tg(Tp1:VenusPEST) embryo treated with DMSO demonstrates Notch activity within the axial and sprouting vasculature (n=6). Embryos were mounted in E3 with tricaine. Treatment began at 18 ss, and continued, at RT, for 15 hours and 10 minutes. See Fig. S3 for still images.