Supplemental Movie 2
Notch signaling is lost in the vasculature upon MAPK inhibition. Representative time-lapse confocal microscopy of a Tg(kdrl:mCherry); Tg(Tp1:VenusPEST) embryo treated with SL327 (30 uM) demonstrates reduced Notch activity within the vasculature, and decreased sprout elongation (n=6). Treatment began at 18 ss, and continued, at RT, for 15 hours and 10 minutes. See Fig. S3 for still images.
Jason E. Fish, Manuel Cantu Gutierrez, Lan T. Dang, Nadiya Khyzha, Zhiqi Chen, Shawn Veitch, Henry S. Cheng, Melvin Khor, Lina Antounians, Makon-Sébastien Njock, Emilie Boudreau, Alexander M. Herman, Alexander M. Rhyner, Oscar E. Ruiz, George T. Eisenhoffer, Alejandra Medina-Rivera, Michael D. Wilson, and Joshua D. Wythe
2017. 144:2428-2444; doi: 10.1242/dev.146050