Supplemental Movie 2

Notch signaling is lost in the vasculature upon MAPK inhibition. Representative time-lapse confocal microscopy of a Tg(kdrl:mCherry); Tg(Tp1:VenusPEST) embryo treated with SL327 (30 uM) demonstrates reduced Notch activity within the vasculature, and decreased sprout elongation (n=6). Treatment began at 18 ss, and continued, at RT, for 15 hours and 10 minutes. See Fig. S3 for still images.