Movie 12

PE of CKO at E9.5 directly contacts the heart, and PE of control at E9.75 directly contacts the heart.
Tbx18Cre/+; Cdc42fl/+; mTmG (control) at E9.75 and Tbx18Cre/+; Cdc42fl/fl; mTmG (CKO) hearts at E9.5 were fixed and whole mount stained for MF20, a marker for cardiomyocyte, or PECAM, a marker for endothelial cells, or PH3, a marker for mitotic cells. Then the embryos were cleared, 3D imaged, and then reconstructed via Imaris. Suppl. Movie 11 shows the PE directly contacting the heart in a CKO heart at E9.5. Suppl. Movie 12 shows the PE directly contacting the heart in a control heart at E9.75.

CDC42 is required for epicardial and pro-epicardial development by mediating FGF receptor trafficking to the plasma membrane

Jingjing Li, Lianjie Miao, Chen Zhao, Wasay Mohiuddin Shaikh Qureshi, David Shieh, Hua Guo, Yangyang Lu, Saiyang Hu, Alice Huang, Lu Zhang, Chen-leng Cai, Leo Q. Wan, Hongbo Xin, Peter Vincent, Harold A. Singer, Yi Zheng, Ondine Cleaver, Zhen-Chuan Fan, and Mingfu Wu

Development 2017. 144:1635-1647; doi: 10.1242/dev.147173