Movie 6

Dorsal side of a control heart at E9.75 is covered by epicardium and ventral side shows some epicardial islands, while the dorsal and ventral sides of a CKO heart were not covered.
Tbx18Cre/+; Cdc42fl/+; mTmG (control) and Tbx18Cre/+; Cdc42fl/fl; mTmG (CKO) hearts at E9.75 were fixed and whole mount stained for ╬▒Tubulin and PH3, a marker for mitotic cells. Then the embryos were cleared and 3D imaged, and then reconstructed via Imaris. Suppl. Movie 5 shows the reconstructed control heart. Suppl. Movie 6 shows the reconstructed CKO heart. The control heart shows more epicardial coverage than the CKO hearts, and some epicardial islands.

CDC42 is required for epicardial and pro-epicardial development by mediating FGF receptor trafficking to the plasma membrane

Jingjing Li, Lianjie Miao, Chen Zhao, Wasay Mohiuddin Shaikh Qureshi, David Shieh, Hua Guo, Yangyang Lu, Saiyang Hu, Alice Huang, Lu Zhang, Chen-leng Cai, Leo Q. Wan, Hongbo Xin, Peter Vincent, Harold A. Singer, Yi Zheng, Ondine Cleaver, Zhen-Chuan Fan, and Mingfu Wu

Development 2017. 144:1635-1647; doi: 10.1242/dev.147173