Movie 9

FGF2 stimulation promotes PECs translocation in ex vivo cultured hearts.
WT1^CreERT2; Cdc42^fl/+; mTmG heterozygous embryos were cultured ex vivo either with vehicle or FGF2 at a concentration of 2 ng/ml medium for 24 hours. Then the embryos were fixed and whole mount stained for MF20, a marker for cardiomyocytes. Then the embryos were cleared and 3D imaged. The heart is shown section-by-section in each movie. The FGF2- treated heart showed more epicardial cells attached to the heart than the vehicle treated heart.