Movie 4

ph-AKT-GFP with and without PDGF-A protein
Ph-AKT-GFP mRNA injected NC cells with and without PDGF-A protein (50ng ml^-1 at t=300sec) addition. PH-AKT-GFP translocates to the Plasma membrane after protein addition, no effect was observed in untreated NC cells, scale bar 10μM (15sec frame^-1).