Movie 1

The Drosophila trachea system forms normally in the absence of MyoII function
Time lapse imaging of sqhAx3/Y; sqh-Sqh-GFP, btl-Gal4, UAS-mCherry_NLS/+ control (left) and sqhAx3/Y; sqh-Sqh-GFP, btl-Gal4, UAS-mCherry_NLS/UAS-deGradFP Sqh-GFP knock-down (right) embryos (10min intervals, 20x objective). deGradFP expression resulted in the spotted accumulation of Sqh-GFP (green, right top panel), indicating deGradFP activity and effective inactivation of MyoII function. Labelling of trachea nuclei by mCherry_NLS (btl::Gal4) shows that despite knock-down of MyoII function in tracheal cells development of the tracheal system is not impaired compared to the control embryo (left).