Movie 2

Gas filling of the tracheal tubes proceeded normally in Sqh-GFP knock-down embryos
Time lapse imaging of sqhAx3/Y; sqh-Sqh-GFP, btl-Gal4, UAS-mCherryNLS/+ control (left) and sqhAx3/Y; sqh-Sqh-GFP, btl-Gal4, UAS-mCherryNLS/UAS-deGradFP Sqh-GFP knock-down (right) embryos (10min intervals, 20x objective). Gas filling of the tracheal tubes (bright field channel, bottom panels) is not impaired by knockdown of Sqh-GFP and occurs similar to control embryos approximately 5 hours past DC.

Myosin II is not required for Drosophila tracheal branch elongation and cell intercalation

Amanda Ochoa-Espinosa, Stefan Harmansa, Emmanuel Caussinus, and Markus Affolter

Development 2017. 144:2961-2968; doi: 10.1242/dev.148940