Movie 2

Gas filling of the tracheal tubes proceeded normally in Sqh-GFP knock-down embryos
Time lapse imaging of sqhAx3/Y; sqh-Sqh-GFP, btl-Gal4, UAS-mCherry_NLS/+ control (left) and sqhAx3/Y; sqh-Sqh-GFP, btl-Gal4, UAS-mCherry_NLS/UAS-deGradFP Sqh-GFP knock-down (right) embryos (10min intervals, 20x objective). Gas filling of the tracheal tubes (bright field channel, bottom panels) is not impaired by knockdown of Sqh-GFP and occurs similar to control embryos approximately 5 hours past DC.