Movie 2

Cells in the medial PPE hardly move from their position. The movie shows an example of an embryo in which a plasmid carrying the photo-convertible Kaede sequence was electroporated at gastrula stage in the median PPE domain. After four hours, few kaede-expressing cells positioned in the median PPE were hit with a laser beam to photoconvert the kaede to red. The behaviour of red-labelled cells was recorded for the next few hours until the embryo reached stage HH8/9.