Movie 1

Time lapse of a subset of a pupal leg disc expressing alphaCat-TagRFP in the ap domain (which encompasses the t4-t5 fold) and the GC3Ai construct to reveal caspase activity and thus visualise apoptotic cell. The apoptotic cell shape can be followed on the apical (top) and sagittal (bottom) sections. Note the reduction of the apical surface of the apoptotic cell on the apical views, the transient deformation of the apical surface on the sagittal views and the formation of apoptotic bodies.

A fluorescent toolkit for spatiotemporal tracking of apoptotic cells in living Drosophila tissues

Sonia Schott, Arnaud Ambrosini, Audrey Barbaste, Corinne Benassayag, Mélanie Gracia, Amsha Proag, Mégane Rayer, Bruno Monier, and Magali Suzanne

Development 2017. 144:3840-3846; doi: 10.1242/dev.149807