Movie 3

Hh and Ptc colocalize in cytoneme-associated motile puncta
Puncta containing BAC-encoded Hh:GFP and BAC-encoded Ptc-mCherry move in cytonemes marked with CD4:IFP2.0-HO1 (ptc-Gal4 UAS-CD4:IFP2.0-HO1; blue) that extend from A compartment cells in the hinge region of a late 3^rd instar wing disc. Movement is in a net retrograde direction towards the A compartment. Pictures were taken every 4 seconds during a 6.5 min period and are shown at 10 frames/second.