Movie 3

Hh and Ptc colocalize in cytoneme-associated motile puncta
Puncta containing BAC-encoded Hh:GFP and BAC-encoded Ptc-mCherry move in cytonemes marked with CD4:IFP2.0-HO1 (ptc-Gal4 UAS-CD4:IFP2.0-HO1; blue) that extend from A compartment cells in the hinge region of a late 3rd instar wing disc. Movement is in a net retrograde direction towards the A compartment. Pictures were taken every 4 seconds during a 6.5 min period and are shown at 10 frames/second.

Essential basal cytonemes take up Hedgehog in the Drosophila wing imaginal disc

Weitao Chen, Hai Huang, Ryo Hatori, and Thomas B. Kornberg

Development 2017. 144:3134-3144; doi: 10.1242/dev.149856