Supplemental Movie 1

Confocal microscopy of E15.5 WT (Supplemental 1) and Y783A (Supplemental 2) mutant kidneys stained with E-cadherin antibodies was performed. Images of kidneys in the same orientation were captured as Z-stacks and the raw confocal images were processed using ImageJ carrying the ND2 plugin to create the 3D re-constructed image file and movie.