Supplemental Movie 4

Confocal microscopy of E12.5 WT (Supplemental 3) and talin1/2 null (Supplemental 4) kidneys stained with E-cadherin antibodies was performed. Images of kidneys in the same orientation were captured as Z-stacks and the raw confocal images were processed using ImageJ carrying the ND2 plugin to create the 3D re-constructed image file and movie.

Talin regulates integrin β1-dependent and -independent cell functions in ureteric bud development

Sijo Mathew, Riya J. Palamuttam, Glenda Mernaugh, Harini Ramalingam, Zhenwei Lu, Ming-Zhi Zhang, Shuta Ishibe, David R. Critchley, Reinhard Fässler, Ambra Pozzi, Charles R. Sanders, Thomas J. Carroll, and Roy Zent

Development 2017. 144:4148-4158; doi: 10.1242/dev.149914