Movie S5

PVDp::AFF-1 L4 regenerates similarly to wild-type
Inverted fluorescence confocal maximum intensity projections time lapse movie of PVDp::AFF-1 (AFF-1 overexpression in the PVD) L4 nematode. The worm was injured and imaged immediately after. In this movie, the PVD rapidly regenerates (within 3 hours from cut) via menorah-menorah fusion, as marked by blue arrows and by novel outgrowth that fuses to the distal cut primary that bridges the gap ( at time 03:00 see connection between 3ry branch pointed by bottom blue arrow and the distal fragment of severed 1ry branch). Red lightning points to injury site (time 0:05). Red arrowhead marks a candelabrum that degenerated during the movie (starting at time 01:45).