Movie S6

PVDp::AFF-1 5d old animal reconnects after cut
Confocal time lapse movie of 5 days old PVDp::AFF-1 transgenic animal, beginning 5 hours post injury (time 5:00). Growth is seen near site of dendrotomy. The primary branch grows toward the distal primary (time 08:40) and reconnects with it 10 hours after injury (blue arrow). After this time point the worm was recovered and imaged 15 hours later (25 hours after cut), where regeneration via novel outgrowth from 1ry to distal fragment of 1ry branch fusion (right blue arrow) and menorah-menorah fusion (left blue arrow) Hyperbranching is clearly seen by comparing the last time lapse images at times 10:00 and 25:00. Red lightning points at injury site.