Movie 6, related to Figure 5.
Cell movement stalls and HCs extend basal protrusions during MyoII inhibition.
XY (top) and XZ (bottom) views of a 10-hour time-lapse movie from an E15 Atoh1Cre*PR; R26RtdTomato explant (two image sequences are spliced together at the 06:15 mark, but all images are of the same explant). 10 μM blebbistatin was added after 3 hours, and diluted to 1 μM at 6 hours. The movement of both SCs and HCs in the apical direction stalls during while MyoII activity is inhibited by blebbistatin treatment, and cells extend numerous thin protrusions, including a basally-directed protrusion from presumptive HC. Images were acquired at 3 min. intervals.