Movie 1

Live imaging of cytonemes of a GFP and CD8-mCherry positive cell. Images of the full axial plane of the cell were taken at 11.245 second intervals. The video was encoded with a 2.4 frame/second rate. GFP (green) and CD8-mCherry (magenta) are located within extensions which originate from regions of the cell not in contact with the slide.

A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport

William J. Bodeen, Suresh Marada, Ashley Truong, and Stacey K. Ogden

Development 2017. 144:3612-3624; doi: 10.1242/dev.152736