Movie 2
Live imaging of cytonemes of a GFP and Disp-mCherry positive cell. Images of the full axial plane of the cell were taken at 13.545 second intervals. The video was encoded with a 2 frame/second rate. In the time lapse, GFP (green) and Disp-mCherry (magenta) cytonemes exhibit slower retraction rates compared to control cells.
A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport
Development 2017. 144:3612-3624; doi: 10.1242/dev.152736