Movie 3

Live imaging of Disp-mCherry puncta in cytonemes. Images of the full axial plane of the cell were taken at 13 second intervals. The video was encoded with a 2 frame/second rate. Disp-mCherry (magenta) puncta travel along cytonemes. Cytoplasmic GFP is green.

A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport

William J. Bodeen, Suresh Marada, Ashley Truong, and Stacey K. Ogden

Development 2017. 144:3612-3624; doi: 10.1242/dev.152736