Ex-vivo video-microscopy tracing of fluorescent micro-beads in the wild-type mouse forebrain.
The original video of an ex-vivo brain slice with addition of fluorescent micro-beads in the P7 Ccdc39wt/wt forebrain ventricle (left). The enlarged and cropped video analyzed in NIS Element software (right). Tracking of the fluorescent micro-beads in the wild-type brain showed that the beads flowed quickly in one direction and exit the field of view very rapidly (video captured at rate of 41 frames per second were played back at a rate of 5 frames per second). Average flow speed was 68 ±0.7 m/sec. Scale bar is 20 μm.
Zakia Abdelhamed, Shawn M. Vuong, Lauren Hill, Crystal Shula, Andrew Timms, David Beier, Kenneth Campbell, Francesco T. Mangano, Rolf W. Stottmann, and June Goto
2018. 145:None-None; doi: 10.1242/dev.154500