Ex-vivo video-microscopy tracing of fluorescent micro-beads in the Ccdc39prh/prh mutant forebrain.
The original video of an ex-vivo brain slice with addition of fluorescent micro-beads in the P7 Ccdc39prh/prh forebrain ventricle (left). The enlarged and cropped video analyzed in NIS Element software (right). Tracking of the fluorescent micro-beads in the Ccdc39prh/prh animals, the beads flowed very slowly in a multiple directions and can be detected in the field of view during the video recording (video captured at rate of 41 frames per second and were played back at a rate of 5 frames per second). Average flow speed was 11 ± 0.07 m/sec. Scale bar is 20 μm.
Zakia Abdelhamed, Shawn M. Vuong, Lauren Hill, Crystal Shula, Andrew Timms, David Beier, Kenneth Campbell, Francesco T. Mangano, Rolf W. Stottmann, and June Goto
2018. 145:None-None; doi: 10.1242/dev.154500