Ex-vivo video-microscopy tracing of fluorescent micro-beads in the Ccdc39prh/prh mutant forebrain.
The original video of an ex-vivo brain slice with addition of fluorescent micro-beads in the P7 Ccdc39prh/prh forebrain ventricle (left). The enlarged and cropped video analyzed in NIS Element software (right). Tracking of the fluorescent micro-beads in the Ccdc39prh/prh animals, the beads flowed very slowly in a multiple directions and can be detected in the field of view during the video recording (video captured at rate of 41 frames per second and were played back at a rate of 5 frames per second). Average flow speed was 11 ± 0.07 m/sec. Scale bar is 20 μm.