Supplemental Movie 1

Example 3D reconstruction of confocal image stacks using Amira software for lung explant presented in Figure 1E. Epithelium is shown in green; skeletonization of major branches is indicated by a blue line for clarity.

Microfluidic chest cavities reveal that transmural pressure controls the rate of lung development

Celeste M. Nelson, Jason P. Gleghorn, Mei-Fong Pang, Jacob M. Jaslove, Katharine Goodwin, Victor D. Varner, Erin Miller, Derek C. Radisky, and Howard A. Stone

Development 2017. 144:4328-4335; doi: 10.1242/dev.154823