Movie 4

Following row mesendoderm cells in controls form dynamic FAs: Time-lapse TIRF images of control following row cells expressing EGFP-paxillin (Green) and LifeAct-mCherry (Red). Time interval between each frame is 15 secs and images were collected for 5 mins (Movie is representative of 9 individual explants imaged across 3 separate experiments). Playback rate is 4 frames per second. Scale bar=25um

Mechanical and signaling roles for keratin intermediate filaments in the assembly and morphogenesis of Xenopus mesendoderm tissue at gastrulation

Pooja R. Sonavane, Chong Wang, Bette Dzamba, Gregory F. Weber, Ammasi Periasamy, and Douglas W. DeSimone

Development 2017. 144:4363-4376; doi: 10.1242/dev.155200