Movie 5

Leading edge cells of krt8 morphant explants form large FAs: Time-lapse TIRF images of krt8 morphant leading edge cells expressing EGFP-paxillin (Green) and LifeAct-mCherry (Red). Time interval between each frame is 30 secs and images were collected for 5 mins (Movie is representative of 9 individual explants imaged across 3 separate experiments). Playback rate is 4 frames per second. Scale bar=25um