Movie 6

Following row cells of krt8 morphant explants form stable FAs: Time-lapse TIRF images of krt8 morphant following row cells expressing EGFP-paxillin (Green) and LifeAct-mCherry (Red). Time interval between each frame is 30 secs and images were collected for 5 mins (Movie is representative of 9 individual explants imaged across 3 separate experiments). Playback rate is 4 frames per second. Scale bar=25um

Mechanical and signaling roles for keratin intermediate filaments in the assembly and morphogenesis of Xenopus mesendoderm tissue at gastrulation

Pooja R. Sonavane, Chong Wang, Bette Dzamba, Gregory F. Weber, Ammasi Periasamy, and Douglas W. DeSimone

Development 2017. 144:4363-4376; doi: 10.1242/dev.155200