Movie 9

Keratin IFs disassemble upon activation of mCherry-PA-Rac1Q61L: Dissociated mesendoderm cells expressing mCherry-PA-Rac1Q61L (Red) and EGFP-krt8 (Green) were irradiated with a 456nm laser. Box indicates region of activation. Cells were irradiated twice at the same region. Confocal images were taken 15 secs apart. Playback time 4 frames per second (Number of cells analysed = 3). Scale bar=25um