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Movie 2

Cortical F-actin in live embryos: effect of cytochalasin D in wild-type. Left: cortical F-actin in wild-type, as in Movie 1 (left) and corresponding to still data in Fig. 6A. Right: F-actin cortex appears degraded, consistent with F-actin depolymerization. Representative movie of 3 trials. In both movies, F-actin is detected by the Life Act transgene, with images at 200x magnification, corresponding to scale bar in Fig. 6.