Movie 1

Calcium image analysis of an early stage wild-type embryo using ratiometric calciumsensitive Fura-2 dye. Imaging was initiated at the 2-cell stage to insure that imaged embryos were fertilized. Images in the movie correspond to embryo in Fig. 1A (second cleavage furrow), and Fig. S1A (third cleavage furrow) after a 90°C counterclockwise rotation. The presented movie is representative of movies in three analyzed wild-type embryos. See Methods, Fig. 2 legend and Fig. S1 legend for details.

Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo

Celeste Eno, Timothy Gomez, Diane C. Slusarski, and Francisco Pelegri

Development 2018. 145:None-None; doi: 10.1242/dev.156604