Calcium image analysis of an early stage nebel mutant embryo using ratiometric calcium-sensitive Fura-2 dye. Imaging was initiated at the 2-cell stage to insure that imaged embryos were fertilized. Images in the movie correspond to embryo in Fig. 1B (second cleavage furrow), and Fig. S1B (third cleavage furrow). The presented movie is representative of movies in six analyzed nebel mutant embryos. See Methods, Fig. 2 legend and Fig. S1 legend for details.