3D reconstruction of structural illumination image stack showing close association between LEC cluster and extravascular RBC in an E13.5 mesentery.
A movie showing 3D reconstruction of an image stack acquired by structural illumination microscopy of whole mount E13.5 R26-mTmG;Vegfr3-CreERT2 mesentery stained for GFP (green), PROX1 (grey; LEC nuclei) or visualized by autofluorescence (grey; RBC). The image stack was acquired at 0.11 μm intervals covering a z distance of 10.1 μm. 3D surface rendering was applied based on signal intensity. A corresponding z-projection is shown in Fig. 1G. The movie is displayed at 25 frames per second.