Movie 4B

Z-stack of confocal images of E14 mesenteric vessels stained for the pan-EC marker PECAM1.
Series of confocal images of E14 mesenteric vessels stained for PECAM1, showing intercellular gaps in the venous endothelium. The movie goes through 28.28 μm distance covering the entire thickness of tissue containing blood vessels (Movie 4A), or 4.2 μm distance covering the venous endothelial layer at the marked position (yellow arrow) to highlight an intercellular gap (Movie 4B). Corresponding z-projections are shown in Fig. 3A (left panels). Images within the series were acquired at 0.28 μm intervals and the movie is displayed at 3 frames per second.