Three-dimensional Imaris rendering of deconvolved 100x confocal z-stack of a normal day 45 neuronal culture of RUES2-22Q stained for MAP2 (green), CTIP2 (purple), nestin (red), and DAPI (blue). Note the thin processes, and the absence of multi-nucleated cells.
Albert Ruzo, Gist F. Croft, Jakob J. Metzger, Szilvia Galgoczi, Lauren J. Gerber, Cecilia Pellegrini, Hanbin Wang, Maria Fenner, Stephanie Tse, Adam Marks, Corbyn Nchako, and Ali H. Brivanlou
Development
2018. 145:None-None; doi: 10.1242/dev.156844