Movie 2

Three-dimensional rendering of 100x confocal z-stack of an abnormal progenitor cell in RUES2-58Q cultures after 45 days of neuronal differentiation, stained for MAP2 (green), CTIP2 (purple), nestin (red), and DAPI (blue). Note the abnormal progenitor cell in the center with an enlarged soma, displaying multiple separated nuclei, thicker processes, enlarged vacuoles and a co-expression of nestin and MAP2 markers.