Movie 3

Three-dimensional rendering of 100x confocal z-stack of abnormal post-mitotic neurons in RUES2-50Q cultures after 45 days of neuronal differentiation, stained for MAP2 (green), CTIP2 (purple), nestin (red), and DAPI (blue). Several multinucleated cells can be observed in the region, but of upmost interest is the cell in the top-right, which displays the typical phenotype of abnormal post-mitotic neurons: multiple nuclei linked together by strands of DNA, forming a ‘nuclear bouquet’.