Movie S2

Spontaneous muscle bundle formation and contractile activity in adherent differentiated ES cultures. Mouse ES cells were differentiated in to PSM-like cells according to the CDL medium protocol and subsequently cultured in HS2%. Large fiber bundle formed spontaneously in vitro anchoring only by extremities (not shown). Note synchronous twitching of the bundle of aligned myofibers. Real time speed.

Recapitulating early development of mouse musculoskeletal precursors of the paraxial mesoderm in vitro

Jérome Chal, Ziad Al Tanoury, Masayuki Oginuma, Philippe Moncuquet, Bénédicte Gobert, Ayako Miyanari, Olivier Tassy, Getzabel Guevara, Alexis Hubaud, Agata Bera, Olga Sumara, Jean-Marie Garnier, Leif Kennedy, Marie Knockaert, Barbara Gayraud-Morel, Shahragim Tajbakhsh, and Olivier Pourquié

Development 2018. 145:None-None; doi: 10.1242/dev.157339