Supplemental Movie 2

Peristalsis of a dissected Ret third instar midgut.
A Ret^LM1 mutant third instar larval midgut dissected and observed. The head is to the left with two metal pins inserted into it. The thin esophagus is visible connecting the head to the proventriculus and pink food is visible immediately posterior in the start of the midgut. Peristaltic contractions are visible in the esophagus, proventriculus and the start of the midgut but fail to propagate as a wave into the rest of the midgut.