Movie 5

Live imaging of border cell clusters expressing different mutant forms of aPKC. Expressing the dominant negative form of aPKC (aPKC-DN) resulted in suppression of leading protrusion formation, even though dynamic filopodial structures were seen forming randomly. In contrast, expression of the overactivated forms of aPKC (aPKC-CAAX and aPKC-CA) resulted in formation of large ectopic protrusions. Their clusters are much less coherent than the wild type clusters, individual border cells appear stretched outward. Scale bars: 10μm.

aPKC is a key polarity determinant in coordinating the function of three distinct cell polarities during collective migration

Heng Wang, Zhiqian Qiu, Zehao Xu, Samuel John Chen, Jun Luo, Xiaobo Wang, and Jiong Chen

Development 2018. 145:None-None; doi: 10.1242/dev.158444