Movie 5

Live imaging of border cell clusters expressing different mutant forms of aPKC. Expressing the dominant negative form of aPKC (aPKC-DN) resulted in suppression of leading protrusion formation, even though dynamic filopodial structures were seen forming randomly. In contrast, expression of the overactivated forms of aPKC (aPKC-CAAX and aPKC-CA) resulted in formation of large ectopic protrusions. Their clusters are much less coherent than the wild type clusters, individual border cells appear stretched outward. Scale bars: 10μm.