Movie 5

A 3D reconstruction of the cardiac outflow region (horizontal rotation) from Tup-GFP stage 16 embryo showing Tup expression (green) in EPCs, heart, amnioserosa and epidermis. Msp300 staining (red) reveals the COM muscle and cardiac cells. Note the position of the most anterior Eve cells (blue) above the COMs attachment to the heart and the entire OHS architecture.