3D movie of 3DISCO-cleared hindbrain from an E13 Ntn1-/- embryo showing the massive invasion of trigeminal and auditory nerves by Robo3-immunoreactive commissural axons. Abbreviations: Ce, cerebellum; Hind, hindbrain; Mes, mesencephalon; Fr; Fasciculus retroflexus; V, trigeminal nerve; VIII, auditory nerve.