Movie 7

3D Movies of an E16 Ntn1^-/- embryo electroporated unilaterally with GFP at E13. Whole-mount immunolabeling for Barhl1 (Green) and Robo3 (Magenta) and 3DISCO clearing. GFP+/Barhl1+ pontine neurons invade the PNS. Abbreviations: Hind, hindbrain; Mes, mesencephalon; Fr; Fasciculus retroflexus; V, trigeminal nerve; VIII, auditory nerve