Movie 2
Dual-reporting hPSCs enable identification of cells during transition from CPC to NkxEC fate. Beginning at 8 days of differentiation, cells expressing both Nkx2.5GFP and VPrmOrange were identified by co-localization masking (shown in white). The plot on the right identifies signal intensity for Nkx2.5GFP and VPrmOrange pixels on the x-axis (1) and y-axis (2), respectively. Pixels expressing both transgenes are designated in field 3.
Wnt inhibition promotes vascular specification of embryonic cardiac progenitors
Development 2018. 145:None-None; doi: 10.1242/dev.159905