Evaluation of EC axial polarization in-vivo
Illustration of how EC polarization was quantified in vivo using individual sections of a z-stack. This example shows a representative control artery at P7. ERG (blue), CD31 (Green), and Golph4 (Red) stainings were combined to evaluate Golgi polarization. Confocal images were acquired at high magnification and the sequential z-slices from different channels were merged into a single hyperstack using FIJI software. Arterial ECs are highly polarized and orient their Golgi in front of the nucleus facing the predicted direction of blood flow (arrows).
Bàrbara Laviña, Marco Castro, Colin Niaudet, Bert Cruys, Alberto Álvarez-Aznar, Peter Carmeliet, Katie Bentley, Cord Brakebusch, Christer Betsholtz, and Konstantin Gaengel
Development
2018. 145:None-None; doi: 10.1242/dev.161182